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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has become noticed in lots of tumors, which leads to chromosome instability, and therefore may possibly lead to tumorigenesis. This protein was located to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a significant job in advertising mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already described. Two additional splice variants had been reported but detailed nucleotide sequence facts just isn't yet offered.

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Blocking peptides are peptides that bind specially to the concentrate on antibody and block antibody binding. These peptide usually contains the epitope regarded by the antibody.

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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...

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This guidebook outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...

Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody vs . the antibody by yourself, one can see which staining is certain.

Goal Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins show unique expression and degradation patterns which add into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates on the G1-S period boundary and is also degraded as cells progress through S phase.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...

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